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. 2021 Apr 6;12:47. doi: 10.1186/s40104-021-00569-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Schematic outline of the experimental schedule. The experimental diets were provided from 7 d post-hatch until the end of the experiment. Chickens were orally challenged with 25× the recommended dose of the coccidiosis vaccine at d 14. Blood, intestine, and meat samples were obtained on d 20 and 35, and body weights were assessed at 7, 20, and 35 d