Fig. 6.

Adoptive transfer of total CD4⁺ T cells from LL-CFA/I-treated SjS mice restores SFR and increases both the frequency and activity of Tregs in recipient diseased SjS mice. a The donor pool composed of SjS females (8/group; 16 wks of age) treated four times at 4 wk intervals with either LL vector or LL-CFA/I similar to that described above. Two wks following the last dose given on wk 28, total LN and splenic CD4⁺ T cells were purified to > 95% purity, and 1 × 10⁶ CD4⁺ T cells/mouse were i.v. injected into recipient SjS females (> 20 wks of age) (10/group). The percentage of CD25⁺CD4⁺ T cells in each group was measured 2 wks later. b Only recipients given CD4⁺ T cells from LL-CFA/I-treated mice showed restored SFR; ***P < 0.0001, ****P < 0.00001 versus LL vector-treated mice. MLN Treg composition as percent CD4⁺ T cells in the recipients are shown in representative FACS plots and histograms: c Foxp3⁺, d IL-10⁺, and e TGF-β⁺. f–h Frequency of CD25⁺CD4⁺ T cell subsets as f CD25⁺CD4⁺, g Foxp3⁺ CD25⁺CD4⁺, h IL-10⁺ CD25⁺CD4⁺, and i TGF-β⁺ CD25⁺CD4⁺ T cells in recipients are depicted; *P < 0.05, **P < 0.01 for comparison between recipients given donor CD4⁺ T cells from LL-CFA/I or LL vector. Data are representative of two experiments