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. 2020 Dec 22;11(1):jkaa005. doi: 10.1093/g3journal/jkaa005

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© The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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The FlyORF-TaDa system generates new lines for cell-type specific profiling. (A) Schematic representation of the FlyORF-TaDa system. When the FlyORF-TaDa donor line is crossed to a FlyORF line and a heat-shock-inducible flippase (hs-Flp), recombination between compatible FRT5 sites generates a new TaDa line for the ORF in the progeny. These lines can be screened for in the subsequent generation by eye color. Dashed lines show GAL4/UAS-induced transcription; *stop codon. (B) Correlation of DNA-binding profiles generated for MRG15 in NSCs using the FlyORF-TaDa system, compared to conventional TaDa (Pearson’s correlation is shown).