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Identification of EVs. a The morphology of EVs was observed under the transmission electron microscopy; b Western blot analysis was utilized to detect levels of surface protein markers, CD9, CD63 and calnexin, in which 1 presented healthy control group, 2 presented benign individuals and 3 presented BC patients; the supernatant after EVs enrichment served as the control; c NTA was used to observe the size and concentration of EVs. Three independent experiments were performed
