Fig. 5.

NEAT1 silencing inhibits EVs-induced promotion of BC cell invasion and migration. a RT-qPCR was applied to detect NEAT1 expression after EV treatment; NEAT1 expression in MCF-7 and MDA-MB-231 cells after treatment of serum EVs from BC patients was upregulated; b RT-qPCR was applied to detect NEAT1 expression in MCF-7 and MDA-MB-231 cells after transfection of NEAT1 siRNA; c transwell assay was utilized to measure MCF-7 and MDA-MB-231 cell invasion; d scratch test was applied to measure MCF-7 and MDA-MB-231 cell migration; e lung metastases in nude mice were applied to detect metastases in vivo. Three independent experiments were performed; * p < 0.05; data in panels a–h were analyzed using two-way ANOVA, and data in panel i were analyzed using one-way ANOVA. Tukey’s multiple comparisons test was used for pairwise comparisons after ANOVA