Fig. 7.

NEAT1 sponges miR-141-3p and KLF12 is the direct target gene of miR-141-3p. a Venn map of predicting miRs downstream of NEAT1 from DIANA tools (blue), miRcode (green) and StarBase (yellow) databases respectively; b RT-qPCR was applied to detect miR-141-3p expression in cells; c Bioinformatics website (http://starbase.sysu.edu.cn/) [45] and dual-luciferase reporter gene assay were applied to confirm the relationship between miR-141-3p and NEAT1; d RNA-pull down assay was used to verify the binding relationship between NEAT1 and miR-141-3p; e Venn map of predicting downstream genes of miR-141-3p from StarBase, Targetscan, miRWalk and miRTarBase databases; f RT-qPCR and Western blot analysis were utilized to detect levels of miR-141-3p and KLF12 in cells after overexpression of miR-141. g Bioinformatics website and dual-luciferase reporter gene assay were applied to confirm the targeting relationship between miR-141-3p and KLF12. Three independent experiments were performed; * p < 0.05; data were analyzed using two-way ANOVA, and Tukey’s multiple comparisons test was used for pairwise comparisons after ANOVA