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. 2021 Jan 11;11(1):jkaa060. doi: 10.1093/g3journal/jkaa060

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© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ura7 and Asn1 cytoophidia in S. cerevisiae. Ura7-GFP Asn1-mCherry cells were grown in rich medium and cells were collected after 6, 24, and 168 h culture. The cells were subsequently fixed by 4% PFA at room temperature for 10 min. Ura7-GFP and Asn1-mCherry protein were observed by fluorescent microscopy. The abundance of cytoplasmic cytoophidia were calculated and the average length of cytoplasmic cytoophidia were plotted. (A) Representative confocal image of Ura7-GFP Asn1-mCherry cells. Ura7 cytoophidia were observed in all three growth phases. Scale bar 5 μm. (B) Quantification of cells with visible cytoophidia was plotted and expressed as percentage of cells containing cytoophidia in three growth phases. *P < 0.05 and ***P < 0.001 (C) The average length of Ura7 and Asn1 cytoophidia was measured and plotted in the stationary phase.