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Primers used for URA7 gene disruption
| Primer name | Sequence (5′ to 3′) |
|---|---|
| URA7 UHA-F | gggGAATTCTTAAAGTTAGCCCTCCCATCTT |
| URA7 UHA-R | gggGGGTACCGTTCTATTGACCAATTCACT |
| URA7 DHA-F | gggTCTAGAATATTTGTAGTGCTTCTCTACAC |
| URA7 DHA-R | gggGAATTCTGATAAATAATCTCCCTGTTCA |
| URA7 KO testing F | AACCACCTGTACGAACTGGCAC |
| URA7 KO testing R | CCAGTTGAAGATGCAAGAACAC |
