Figure 1.

BMP7 peptide library screening on primary human OA articular chondrocytes (OA-HACs)

(A) Schematic representation of the design of the human BMP7 peptide library. (B) COL10A1 mRNA expression in samples from the library screening on a pool of 18 OA-HACs individually exposed for 24 h to the peptides (100 nM) in the library. Data are shown as compared to the control condition (white bar) and full-length BMP7 (1 nM; gray bar). The two blue bars represent candidate peptides p[63−82] and p[113−132]. Data from these two peptides showed significantly downregulated COL10A1 expression but also showed reduced expression of RUNX2, ALPL, COX-2, IL-6, ADAMTS5, and MMP13 and increased expression of SOX9, COL2A1, and NKX3-2 in all tested peptide concentrations. Error bars represent mean ± SEM, and statistical significance for peptide condition or BMP7 versus control condition as determined by unpaired two-tailed Student’s t test is represented as ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; NS, not significant. Data for other measured mRNAs and peptide concentrations are provided in Figure S2.