Figure 1.

Experimental evolution scheme. Colors indicate environmental and reproductive system regimes: black for derivation of the hybrid androdiecious (hermaphrodite/male) population A0 from inbred founders, red for domestication under mixed selfing and outcrossing for 140 generations (resulting in the A6140), orange for continued evolution in the standard environment (three lineages, sampled at 50 and 100 generations), green for evolution in changing environments under androdioecious, trioecious (male/female/hermaphrodite) or monoecious (hermaphrodite) mating systems (50 generations). In each regime, samples from replicate populations (numbered in boxes) are periodically frozen for contemporaneous characterization of ancestral and derived characters. CeMEE RILs were derived from A6140 and all descendant lineages shown.