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. 2020 Aug 26;31(3):231–242. doi: 10.1093/glycob/cwaa082

Fig. 1.

Fig. 1

ELISA in vitro binding assay of Siglec-11 to polySia. (A) Experimental scheme for (B–F). PolySia (0~1 mg/mL) was immobilized on the plate and analyzed binding of (B) mab 735, (C) hSiglec-11(4D)-Fc (-) and Fc (…) to the immobilized polySia. PolySia (1 mg/mL) was immobilized on the plate and binding of (D) Fc, hSiglec-11(5D)-Fc, cSiglec-11(5D)-Fc and hSiglec-11(4D)-Fc, (E) Fc, hSiglec-11(4D)-Fc, hSiglec-11(4D;R120A)-Fc and hSiglec-11(4D;R120K)-Fc to immobilized polySia was analyzed. (F) PolySia (1 mg/ml) was immobilized on the plate and binding of hSiglec-11(4D)-Fc was analyzed with the presence of different concentrations of preincubated free polySia (0-5 mg/mL) or HA (5 mg/mL). n = 3, *P < 0.05 **P < 0.005.