Fig. 3.

Full length Siglec-11 analysis by western blotting. HEK293 cells were transiently transfected with V5-tagged hSiglec-11 for 3 days and cell lysates were analyzed by western blotting. (A) Denature temperature of cell lysate were changed under the reducing condition with 2-ME and analyzed by western blotting with anti-V5 tag antibody. (B and C) Cell lysates were denatured at 4°C for 30 min under reducing (B) and nonreducing condition (C) and analyzed by Western blotting with anti-V5 tag antibody.