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. 2020 Aug 26;31(3):231–242. doi: 10.1093/glycob/cwaa082

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Fig. 7 — Siglec-11 is localized in lipid rafts. (A) Cell lysates of V5-tagged Siglec-11 stably transfected HEK293 cells were analyzed by western blotting with anti-V5 tag antibody. (B) Same cell lysates were fractionated by density gradient separation and analyzed by western blotting with anti-V5 tag antibody. hSiglec-11(5D) (top panel), hSiglec-11(4D) (middle panel) and cSiglec-11(5D) (lower panel) were analyzed. β-actin was used as loading control and flotillin-1 was used as a lipid raft marker.