Table I.

Two approaches to study glycogene function

	Validated guides	Forward genetic screen
Advantage	• Requires less bioinformatics knowledge	• sgRNA libraries can be rapidly designed using established bioinformatics pipelines
	• Good approach when there is one or a few targets to study. Well suited for hypothesis validation	• Same library can be reused for multiple screens. Ideal for hypothesis generation
Disadvantage	• Guide validation can be expensive and time consuming as individual genes have to be screened	• Quality of results depends on the quality of sgRNA
	• Guide validated in one system may not work in others due to epigenetic differences	• Some genes may be missed when library size is large

sgRNA, single-guide RNA.