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. Author manuscript; available in PMC: 2021 Apr 6.
Published in final edited form as: Sci Signal. 2020 Jun 16;13(636):eaay1451. doi: 10.1126/scisignal.aay1451

Fig. 4: Evolution of apoptotic chemical vulnerabilities in cell culture conditions measured by HT-DBP.

Fig. 4:

(A) Comparison of freshly isolated MMTV-PyMT tumors with cells cultured ex vivo for one month. Compounds that inhibit HSP90 are colored blue. Compounds that inhibit mTOR are colored green. Grey dotted lines indicate 3 standard deviations of DMSO treated wells. R2 = 0.38 by Pearson analysis. Data are means of two independent screens. (B) Identity of compounds that primed the freshly isolated tumor only, the cell line only, or both. This analysis only evaluated compounds that did not prime healthy cells for apoptosis. (C to E) Comparison of delta priming by (C) 17-DMAG, (D) AZD2014, and (E) navitoclax on freshly isolated MMTV-PyMT tumor cells cultured ex vivo for one month. Each point represents an independent experiment. Lines represent mean of N = 2 experiments. (F and G) Comparison of drug-induced delta priming in (F) COCA74P and (G) COCA61 cells at days 1 and 8 after tumor extraction. Data are means of triplicates.