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Visual detection at 16, 22 and 30 min of LAMP reaction with 1 × 104 copies of synthetic SARS-CoV-2 RNA (five replicates) or nontemplate control (one replicate), for each set of N and ORF1ab primers, with or without a pretreated saliva sample. The assay was conducted in eight-well PCR strips and the image has been taken from the front of the strips.
