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. 2021 Apr 6:10.2144/btn-2020-0159. doi: 10.2144/btn-2020-0159

Table 1. Primers for SARS-CoV-2 detection.

Primer Sequence (5′ to 3′)
N
F3 TGGCTACTACCGAAGAGCT
B3 TGCAGCATTGTTAGCAGGAT
FIP (Fic + F2) TCTGGCCCAGTTCCTAGGTAGTCCAGACGAATTCGTGGTGG
BIP (B1c + B2) AGACGGCATCATATGGGTTGCACGGGTGCCAATGTGATCT
LF GGACTGAGATCTTTCATTTTACCGT
LB ACTGAGGGAGCCTTGAATACA
ORF1ab
F3 CCTCAACTTGAACAGCCCT
B3 CGAAGAAGAACCTTGCGGT
FIP (Fic + F2) GCCTTCGAGTTCTGCTACCAGCTCATCAAACGTTCGGATGCT
BIP (B1c + B2) ACGGTCGTAGTGGTGAGACACTAAGCCACTGGTATTTCGCC
LF ACATGACCATGAGGTGCAGTTCG
LB TGGTGTCCTTGTCCCTCATGTG

B3: Outer backward primer; BIP: Backward inner primer; F3: Outer forward primer; FIP: Forward inner primer; LB: Loop backward primer; LF: Loop forward primer.