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. 2021 Mar 23;11:648052. doi: 10.3389/fonc.2021.648052

Figure 4.

Figure 4

Overexpression of β-catenin retrieves DHC-induced cell proliferation inhibition. (A) The protein expression of β-catenin in β-catenin/vector overexpressed A375 and MV3 cells treated with 100 μM DHC for 48 h. (B) Cell viabilities of β-catenin/vector overexpressed A375 and MV3 cells treated with 100 μM DHC for 48 h. (C) EdU staining of β-catenin/vector overexpressed A375 and MV3 cells treated with 100 μM DHC for 48 h. (D, E) Cell cycle analysis of β-catenin/vector overexpressed A375 and MV3 cells treated with 100 μM DHC for 48 h. (F, G) The protein and mRNA levels of cyclin D1, c-Myc, and cyclin A2 in β-catenin/vector overexpressed A375 and MV3 cells treated with 100 μM DHC for 48 h. *P < 0.05; **P < 0.01; ***P < 0.001.