FIGURE 4.
ASFV MGF-505-7R targets at STING level. (A and B) Effects of MGF-505-7R on cGAS-STING–triggered IFN-β promoter (A) and ISRE (B) activation. 293T cells (1 × 105) were transfected with IFN-β or ISRE reporter (0.1 μg) and expression plasmids for MGF-505-7R and the indicated plasmids (0.1 μg each). Luciferase assays were performed 24 h after transfection. (C) MGF-505-7R locates at cytoplasm in HeLa cells. HeLa cells were transfected with MGF-505-7R for 24 h. Then, cells were treated with B-DNA for 12 h, fixed, and then stained with DAPI and anti–MGF-Flag Ab. Finally, the location of cells was observed by confocal microscopy. (D) The effects of STING on MGF-505-7R in PK-15 cells. PK-15 or STING knockout PK-15 cells were transfected with MGF-505-7R (2 μg) for 24 h. Then, the cells were retransfected with B-DNA (1 μg/ml) for 12 h before RT-PCR experiments were performed with the indicated primers. Expression of STING and MGF-505-7R was analyzed by Western blot. All the experiments were repeated three times with similar results. Data are shown as means ± SD; n = 3. **p < 0.01. KO, knockout; Luc, luciferase; 7R, Flag-MGF-505-7R; WT, wild-type.