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. 2021 Jan 27;72(8):3012–3027. doi: 10.1093/jxb/erab035

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — The major 45S rDNA locus-carrying chromosome arm shows increased chiasmata in asy1 but not in pch2 or the WT. (A) Top: B. rapa chromosomes organized by size with 5S and 45S rDNA clusters detected by FISH. Bottom: a representative WT cell after FISH. (B) Percentage of #3 rod bivalents with a chiasma in the 45S-carrying arm (‘45S yes’, example FISH image right) or in the opposite arm (‘45S no’, example FISH image left). Total number of rod bivalents scored: WT 41, pch2 16, asy1 36. FISH images: 5S (red), 45S (green); DNA is counterstained with DAPI and shown in blue.