Figure 3.

TNB-486 mediates T cell activation, proliferation, and cytotoxic granule release. CD19+ RI-1 tumor cells were incubated with T cells from a single healthy donor at an E:T ratio of 10:1 and serially diluted indicated test antibody (a) Activation of CD4+ or CD8+ T cells were determined by flow cytometric measurement of the activation marker CD69 after 24 hours of co-culture (b) T cell proliferation was measured by labeling T cells with CFSE and monitoring CD4 or CD8 proliferation after 5 days of co-culture (c) Perforin and granzyme B levels were measured in the cell culture supernatants of co-culture after 48 hours by ELISA