Table 2.

TNB-486 induces lower cytokine release compared to the PC. The TDCC of TNB-486 was assessed as described in Figure 5. Supernatants from the co-culture were used to measure cytokine release. The EC₅₀s of cytokine release are shown in Table 2(A) and the maximum cytokine released is shown in Table 2(B)

(A) EC50 values of dose curves for TNB-486 or PC mediated cytokines
	EC50 (pM), TNB-486						EC50 (pM), PC
Target Cell	RI-1			Raji			RI-1			Raji
Donor ID	D2546	D2921	D0628	D2546	D2921	D0628	D2546	D2921	D0628	D2546	D2921	D0628
IFNγ	1534	N/A	1411	N/A	2164	358	7	N/A	5	8	3	5
IL-2	5364	N/A	6192	N/A	1410	570	18	31	17	3	3	4
IL-6	3487	N/A	2961	N/A	N/A	N/A	15	N/A	10	N/A	N/A	N/A
IL-10	1423	735	1369	N/A	N/A	322	3	N/A	N/A	16	N/A	N/A
TNF	2620	708	3051	N/A	1225	490	18	14	13	1	2	N/A

(B) Maximum cytokine levels induced by TNB-486 or PC (related to Figure 5)
	Maximum Induction (pg/mL), TNB-486						Maximum Induction (pg/mL), PC
Target Cell	RI-1			Raji			RI-1			Raji
Donor ID	D2546	D2921	D0628	D2546	D2921	D0628	D2546	D2921	D0628	D2546	D2921	D0628
IFNγ	42492	13647	24080	18188	18373	6416	65646	22860	28701	80441	43053	17758
IL-2	2061	443	2094	4929	2456	4494	17874	4537	12430	21217	8567	10378
IL-6	246	3	193	39	47	19	1124	131	554	189	79	27
IL-10	1294	74	1804	326	56	213	1448	101	1795	910	99	360
TNF	1473	632	1372	742	500	500	8174	2533	4367	5747	1828	1159