KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal anti-B220 APC-Cy7 (clone RA3-6B2) | BioLegend | Cat# 103224; RRID:AB_313007 |
| Rat monoclonal anti-CD3 eFluor 450 (clone 17A2) | eBiosciences | Cat# 48-0032-82; RRID:AB_1272193 |
| Rat monoclonal anti-CD16/CD32 (clone 2.4G2) | Bio X Cell | Cat# CUS-HB-197; RRID:AB_2687830 |
| Rat monoclonal anti-CD19 PE (clone 6D5) | BioLegend | Cat# 115507; RRID:AB_313642 |
| Rat monoclonal anti-CD38 PerCP-Cy5.5 (clone 90) | BD Biosciences | Cat# 562770; RRID:AB_2737782 |
| Rat monoclonal anti-CD138 APC (clone 281-2) | BD Biosciences | Cat# 558626; RRID:AB_1645216 |
| Rat monoclonal anti-CD138 PE (clone 281-2) | BD Biosciences | Cat# 553714; RRID:AB_395000 |
| Rat monoclonal anti-CD184 PE (clone 2B11) | BD Biosciences | Cat# 551966; RRID:AB_394305 |
| Rat monoclonal anti-CD184 BV421 (clone 2B11) | BD Biosciences | Cat# 562738; RRID:AB_2737757 |
| Mouse monoclonal anti-CXCL12/SDF-1 (clone 79014) | R&D Systems | Cat# MAB310500; RRID:AB_2276927 |
| Mouse monoclonal anti-CXCL12/SDF-1 (clone 79018) | R&D Systems | Cat# MAB350100; RRID:AB_2088149 |
| Mouse monoclonal IgG1 Isotype Control (clone 11711) | R&D Systems | Cat# MAB002; RRID:AB_357344 |
| Goat polyclonal anti-CXCL12/SDF-1 Biotinylated | R&D Systems | Cat# BAF310; RRID:AB_356384 |
| Rat monoclonal anti-Ig kappa light chain PE (clone 187.1) | BD Biosciences | Cat# 559940; RRID:AB_397384 |
| Rat monoclonal anti-Ki-67 Alexa Fluor 647 (clone 16A8) | BioLegend | Cat# 652408; RRID:AB_652408 |
| Mouse monoclonal anti-MHC Class II I-Ab PE (clone AF6-120.1) | eBiosciences | Cat# 12-5320-82; RRID:AB_2572619 |
| Goat polyclonal anti-Mouse IgG (H+L) HRP | Jackson ImmunoResearch | Cat# 115-035-146; RRID:AB_2307392 |
| Rat monoclonal anti-VLA-4 (clone PS/2) | Bio X Cell | Cat# BE0071; RRID:AB_1107657 |
| Chemicals, peptides, and recombinant proteins | ||
| AMD 3100 octahydrochloride | Tocris Bioscience | Cat# 3299 |
| Bovine Serum Albumin, Fraction V (Modified Cohn) | Sigma-Aldrich | Cat# 12-660-100GM |
| BOP | Tocris Bioscience | Cat# 6047 |
| EDTA (0.5M), pH 8.0, RNase-free | Thermo Fisher Scientific | Cat# AM9261 |
| Fibronectin, Bovine Plasma | Sigma-Aldrich | Cat# 341631-1MG |
| Fingolimod (FTY720) | Cayman Chemical | Cat# 10006292 |
| GIBCO Fetal Bovine Serum | Thermo Fisher Scientific | Cat# 10-437-028 |
| Imject Alum Adjuvant | Thermo Fisher Scientific | Cat# 77161 |
| Isothesia (Isoflurane) Solution | Covetrus | Cat# 029405 |
| Lactated Ringer’s Solution | Henry Schein | Cat# 14792 |
| Lonza ACK Lysing Buffer | Thermo Fisher Scientific | Cat# BW10548E |
| Lipopolysaccharides from Escherichia coli O111:B4 | Sigma | Cat# L-2630 |
| NP(24)-KLH | Biosearch Technologies | Cat# N-5060-25 |
| PBS, 10X Powder, pH 7.4 | Thermo Fisher Scientific | Cat# BP665-1 |
| Pertussis Toxin | Sigma-Aldrich | Cat# 516560 |
| Polyinosinic-polycytidylic Acid (Poly(I:C)) | Invivogen | Cat# tlrl-picw |
| Qtracker 655 Vascular Labels | Thermo Fisher Scientific | Cat# Q21021MP |
| Qtracker 705 Vascular Labels | Thermo Fisher Scientific | Cat# Q21061MP |
| Recombinant Murine IL-4 | PeproTech | Cat# 214-14 |
| Recombinant Mouse CXCL12/SDF-1 Alpha | R&D Systems | Cat# 460-SD-050 |
| Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein | R&D Systems | Cat# 796-IC-050 |
| Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein | R&D Systems | Cat# 643-VM-050 |
| RPMI 1640 Medium | Thermo Fisher Scientific | Cat# SH3009601 |
| TMB | MilliporeSigma | Cat# 613544 |
| 2-Mercaptoethanol | Thermo Fisher Scientific | Cat# 21985023 |
| 4-Hydroxytamoxifen | Sigma-Aldrich | Cat# H7904 |
| Critical commercial assays | ||
| BD Cytofix/Cytoperm Fixation/Permeabilization Kit | BD Biosciences | Cat# 554714 |
| Corning Transwell polycarbonate membrane cell culture inserts | Sigma-Aldrich | Cat# CLS3421 |
| High-Capacity RNA-to-cDNA Kit | Applied Biosystems | Cat# 4388950 |
| Live/Dead Fixable Aqua Dead Cell Stain Kit | Thermo Fisher Scientific | Cat# L34957 |
| RNeasy Plus Mini Kit | QIAGEN | Cat# 74134 |
| TaqMan Universal Master Mix II, with UNG | Applied Biosystems | Cat# 4440046 |
| TaqMan Gene Expression Assay (FAM) [CXCL12; ActB] | Applied Biosystems | Cat# 4331182 |
| UltraComp eBeads Compensation Beads | Thermo Fisher Scientific | Cat# 01-2222-41 |
| Experimental models: organisms/strains | ||
| Mouse: APRIL-KO | The Jackson Laboratory | JAX stock# 022971 |
| Mouse: Blimp1-YFP | The Jackson Laboratory | JAX stock# 008828 |
| Mouse: B1-8hi | The Jackson Laboratory | JAX stock# 007775 |
| Mouse: C57BL/6 | Charles River Laboratories | NCI stock# 556 |
| Mouse: C57BL6/J | The Jackson Laboratory | JAX stock# 000664 |
| Mouse: CD4-Cre | The Jackson Laboratory | JAX stock# 022071 |
| Mouse: Cxcl12DsRed | The Jackson Laboratory | JAX stock# 022458 |
| Mouse: UBC PA-GFP | The Jackson Laboratory | JAX stock# 022486 |
| Mouse: Rosa26-CreERT2 | The Jackson Laboratory | JAX stock# 008463 |
| Mouse: Rosa26Stop-tdTomato (AI14) | The Jackson Laboratory | JAX stock# 007914 |
| Oligonucleotides | ||
| Common forward primer for Cxcl12: CCA ACC TGT GCC CTT CAG ATT G | Integrated DNA Technologies | N/A |
| Reverse primer for Cxcl12 isoform α: CAT ATG CTA TGG CGG AGT GTC | Integrated DNA Technologies | N/A |
| Reverse primer for Cxcl12 isoform β: CTT CAG CCC TGC TCA GGA GC | Integrated DNA Technologies | N/A |
| Reverse primer for Cxcl12 isoform γ: ACT GCG GTC CAT CGG CAG G | Integrated DNA Technologies | N/A |
| Software and algorithms | ||
| Adobe After Effects | Adobe | N/A |
| Adobe Illustrator | Adobe | RRID: SCR_010279 |
| Excel | Microsoft | RRID: SCR_016137 |
| FlowJo | Treestar | RRID: SCR_008520 |
| Imaris | Bitplane | RRID: SCR_007370 |
| MATLAB | MathWorks | RRID: SCR_001622 |
| Prism | Graphpad | RRID: SCR_002798 |
| Python | Python | RRID: SCR_008394 |
| Volocity | Quorum Technologies | RRID: SCR_002668 |