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. 2021 Mar 23;11:641174. doi: 10.3389/fcimb.2021.641174

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Copyright © 2021 Brusini, D’Archivio, McDonald and Wickstead

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of novel KKIP-interacting proteins. First and second principal components (PC) of integrated spectral intensities [i.e. the normalized cumulative fragment ion intensity for all significantly identified peptides from each protein [SINQ]; (Trudgian et al., 2011)] for 1520 trypanosome proteins identified in one or more immuno-purifications with YFP-labelled KKIP1-7 (without cross-linking). Previously identified kinetochore proteins (KKT and KKIP) and near-neighbors to the KKIP2,3,4,6 cluster are highlighted. Ribosomal proteins are highlighted as examples of high-abundance negative controls. Eight close neighbors tagged in this study are also indicated.