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. 2021 Mar 23;11:641174. doi: 10.3389/fcimb.2021.641174

Figure 2.

Figure 2

KKIP-interacting proteins include new kinetochore components. Micrographs of native fluorescence in bloodstream-form trypanosomes expressing either known kinetochore components KKT2 and KKIP1 (A), newly identified KKIPs (B), or other proteins detected as co-purifying with KKIPs (C). All proteins are tagged at their N-termini and native fluorescence from mScarlet-I (magenta) or YFP (yellow) is shown. Counter-staining of DNA with 4′,6-diamidino-2-phenylindole (DAPI; cyan) and phase-contrast images are also shown. Representative images from cells in G2, metaphase and anaphase are shown for each cell line. Scale bar: 2 µm. (D) Predicted protein architectures for new (KKIP8-12) and previously identified (KKIP1-7) KKT-interacting proteins. Pfam domains with expectation values ≤ 10-3 and possible regions of coiled-coil (ncoils; p ≥ 0.5, minimum length 8, window size 21) are highlighted.