FIGURE 1.

Methodology and EV separation. [(a) EVs were separated from H9 and U937 culture‐conditioned media by a combination of ultrafiltration and size exclusion chromatography (SEC EVs) or by differential ultracentrifugation (100K EVs). (b) Immunoblots of cell lysates from H9 and U937, EVs separated by ultracentrifugation (100K EVs) and SEC (SEC EVs), and later fractions of SEC (enriched for protein; SEC‐P). Antibodies are specified in Table 1; see also Supplementary Figure 1. (c) Electron micrograph of SEC EVs and 100K EVs from both cell lines. As indicated for each subpanel, leftmost scale bars represent 500 nm at magnification 40,000×; rightmost scale bars are 100 nm at magnification 100,000×. (d) EM of SS and PS. Leftmost scale bars are 500 nm at magnification 17,500×; rightmost scale bars are 100 nm at magnification 65,000×.]