(A and B) Increased luminescent signal was apparent in ipsilateral knees of the INJ group on days 1, 3, 7 and 70 following joint injury, with no changes in contralateral limbs. (C) Mice in the INJ group had persistent increased sensitivity in the hindpaw following injury. (D) Increased NF-κB signaling in joints was associated with reduced withdrawal thresholds in the first week of the study. (E) Mice in the INJ group also had a reduced threshold to noxious mechanical stimuli following joint injury. (F) Static weight bearing was altered up to one week following joint injury. (G) Mice in the INJ group exhibited a trend towards reduced activity, illustrated by animal matched representative traces (H), 2 days post joint injury. CTRL n=5, INJ n=6, *=p<0.05.