Fig 4. Distance matrix of pairwise comparison of G. duodenalis assemblage B isolates of consecutive samples to identify repeated sampling from chronic cases and possible occurrence of transmission chains.

Comparison of concatenated sequences of a common typing scheme (TPI-BG-GDH, 1358 bp) for assemblage B isolates of collection 2 are presented as distance matrix showing the number of pairwise differences in single nucleotide positions. Phylogram of neighbour-joining analysis (only bootstrap values above 50 are shown) is included for illustration. Samples from chronically infected patients were highlighted in the same colour if they met the outlier detection cut-off (mean minus 2SD). Note, five samples revealed identical nucleotide composition suggesting a before unnoticed epidemiological link (black frame). Note furthermore the subsets of isolates around BIII- and BIV-references possessing lower number of variations in single nucleotide positions. Scale bar denotes substitutions per site.