Fig 6. Cytokine gene expression in the cecal tonsils of chickens vaccinated in-ovo with CNP.
At embryonic d18, broiler eggs were vaccinated with either PBS (control) or 1000 μg CNP vaccine. At d7 of age, birds were challenged with live SE (1.0 × 109 CFU/bird). Cecal tonsil samples were collected at 2wk post-challenge and analyzed for cytokine mRNA amounts by RT-PCR. Data represented fold change compared to control. A–IL-1β mRNA; B–IL-10 mRNA; C–iNOS mRNA. Data analyzed by parametric Student t-test; Means+SEM.Bars. n = 6. "*" means P<0.05.