Table 3. Number of significant genes due to stage, treatment, and treatment-by-stage interactions stages 21, 25, 31, and 35 of F. heteroclitus embryo development.

Stage	Population	Treatment	Population X Treatment	Total
21	28	93	24	143
25	24	21	32	77
31	72	157	90	299
35	83	232	21	308

The highest total number of significant genes due to population-by-treatment effects is at stage 31 (90 genes), while the highest numbers of significant genes due to population alone and treatment alone is at stage 35 (83 genes for population, and 232 genes for treatment).