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Extracted chromatograms highlighting histidine-phosphorylated peptides (indicated in color) corresponding to the elution fractions after enrichment using (a) MIP3 and (b) NIP3, and (c) no enrichment of a 1:1:1 BSA/β-Casein/myoglobin digest (the range for relative abundance is from 0 to 40 on the left and from 0 to 2 in the right).
