Table 1. Parameters of the Anisotropy Decays of ATTO-488, StnI (degree of labeling of 2%), in the Absence and Presence of Lipids (1:1:1 DOPC:eSM:Chol with an L:P molar ratio of 160; 4:1 POPC:PSM with an L:P molar ratio of 160)a.
| r1 | θ1 (ns) | r2 | θ2 (ns) | r∞ | |
|---|---|---|---|---|---|
| ATTO-488 | 0.125 ± 0.005 | 0.228 ± 0.012 | – | – | 0.000 ± 0.0004 |
| ATTO-488 with DOPC/eSM/Chol | 0.135 ± 0.006 | 0.232 ± 0.012 | – | – | 0.001 ± 0.0004 |
| StnI | 0.200 ± 0.008 | 0.299 ± 0.030 | 0.089 ± 0.006 | 2.851 ± 0.530 | 0.014 ± 0.002 |
| StnI with DOPC/eSM/Chol | 0.195 ± 0.009 | 0.267 ± 0.034 | 0.088 ± 0.004 | 2.979 ± 0.587 | 0.040 ± 0.003 |
| StnI with POPC/PSM | 0.157 ± 0.007 | 0.224 ± 0.020 | 0.103 ± 0.005 | 2.273 ± 0.170 | 0.031 ± 0.002 |
The initial anisotropies (ri) and correlation times (θi) of each component together with the limiting anisotropy (r∞) are indicated. Values were obtained from fitting, and errors from bootstrap analysis.