Skip to main content
. 2021 Jan 14;60(4):314–323. doi: 10.1021/acs.biochem.0c00840

Table 1. Parameters of the Anisotropy Decays of ATTO-488, StnI (degree of labeling of 2%), in the Absence and Presence of Lipids (1:1:1 DOPC:eSM:Chol with an L:P molar ratio of 160; 4:1 POPC:PSM with an L:P molar ratio of 160)a.

  r1 θ1 (ns) r2 θ2 (ns) r
ATTO-488 0.125 ± 0.005 0.228 ± 0.012 0.000 ± 0.0004
ATTO-488 with DOPC/eSM/Chol 0.135 ± 0.006 0.232 ± 0.012 0.001 ± 0.0004
StnI 0.200 ± 0.008 0.299 ± 0.030 0.089 ± 0.006 2.851 ± 0.530 0.014 ± 0.002
StnI with DOPC/eSM/Chol 0.195 ± 0.009 0.267 ± 0.034 0.088 ± 0.004 2.979 ± 0.587 0.040 ± 0.003
StnI with POPC/PSM 0.157 ± 0.007 0.224 ± 0.020 0.103 ± 0.005 2.273 ± 0.170 0.031 ± 0.002
a

The initial anisotropies (ri) and correlation times (θi) of each component together with the limiting anisotropy (r) are indicated. Values were obtained from fitting, and errors from bootstrap analysis.