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(left) Superposition of the upper vestibule of the WT model (green) and the R207Q mutant (magenta), with relevant residues represented as sticks. (right) PMF profiles for WT (green) and the R207Q (magenta) systems; the pore axis is oriented from the extracellular to the intracellular space, and the positions of the Cα atoms of relevant residues are represented as gray lines.
