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. 2021 Apr 2;10(1):651–663. doi: 10.1080/22221751.2021.1903343

Figure 2.

Figure 2.

Clinical analysis of VSV-EBOV-inoculated pigs. (A) Average daily temperatures of VSV-EBOV-inoculated pigs compared to uninoculated controls. (B) Average lesion scores of VSV-EBOV-inoculated pigs compared to uninoculated controls on each day of the study. (C) Summary of RT-qPCR analysis performed to detect viral RNA in clinical samples collected throughout the study, indicating the presence (+) or absence (−) of viral RNA. P-LN – parotid lymph node; RP-LN – retropharyngeal lymph node; SC-LN – superficial cervical lymph node; M-LN – mandibular lymph node; NP – nasal planum; NA – Not Applicable because not present or collected; DPI - days post inoculation in which positive samples were detected. (D) Representative pictures showing vesicular lesions in VSV-EBOV-inoculated pigs. (E) Immunohistochemistry analysis performed on foot lesion tissue (hoof and skin) collected on 10 DPI necropsy from VSV-EBOV-inoculated pig #15 showing positive (red) immunostaining using anti-EBOV-GP rabbit polyclonal antibody localized to the stratum spinosum and granulosum of the epidermis.