Table 2. List of Peptides Used for CESs and AADAC in Multiple Speciesa.
| enzyme | peptides sequences | species (UniProt ID) |
|---|---|---|
| CES1 | SYPIVNVSK*392 | rabbit (P12337) |
| FWANFAR*505,503,468 | human (P23141), rat (Q63108), pig (F1RF16) | |
| CES2 | NIAHFGGNPGR*194 | rabbit (G1T7P3) |
| ADHGDELPFVFR*466 | human (O00748) | |
| AGVHTFLGIPFAK*67 | rat (A0A0G2K455) | |
| CES3 | LAFPEATEEEK490 | human (Q6UWW8), rabbit (G1SNB1) |
| ATGPETAQPEVDTALGR*42 | rabbit (G1SNB1) | |
| TIASYTVDGTFFPK322 | pig (I3LEI5) | |
| EATQPEVDTTLGR*42 | ||
| NTIYPLTVDGTVFPK*323 | human (Q6UWW8) | |
| TPEEILAEK*338 | rat (P16303) | |
| FAPPQPAEPWNFVK*78 | ||
| AADAC | FWSEYFTTDR258,258,257 | human (P22760), pig (A0A287AXR4), rat (Q9QZH8) |
| YPGFLDVR*318,318,317 | human (P22760), pig (A0A287AXR4), rabbit (Q7M370) | |
| TPTPGSLELAQK*309 | rat (Q9QZH8) | |
| LDVVVVSTNYR145 | rabbit (Q7M370) | |
| Na+/K+ATPase | AAVPDAVGK* | all species |
a
The bold K/R indicate the 13C- and 15N-labeled residues required for quantitation. The number is the C-terminal residue of the peptide in the respective protein, or when the peptide is common for multiple species, the number is for the human isoform. SpikeTides_TQL peptides were used as internal standards. The C-terminal tetrapeptide tag on these is cleaved by the protease digestion. A peptide cocktail (20 nM each) used for simultaneous MRM quantitation was prepared in 0.1 M ammonium bicarbonate solution and ACN (1:4). Peptides marked with an asterisk (*) were used for protein quantitation. Note: the pig CES2 gene is absent from the reference genome [UCSC Genome Browser assembly ID: susScr11].