Table 3. Kinetics for CES Mediated Hydrolysis Reactions.
| substrate | main enzyme | Km (μM) literature data | references | Km(μM) (this study) |
|---|---|---|---|---|
| NPA | multiple esterases | 198 ± 17 (HLM)a | (23, 32, 49, and 63) | 125 ± 13 in pig cornea |
| 68 ± 15 (rabbit cornea) | 263.2 ± 15 in rabbit cornea | |||
| 520 ± 60 (pig liver CES1) | ||||
| DME | CES1 | ≈5 (rhCES1) | (13) | 2.7 ± 1.3 in pig cornea |
| 2.5 ± 0.8 in rabbit cornea | ||||
| FDA | CES2 | 4.87 ± 0.51 (HLM) | (16, 49, and 64) | 16 ± 1.6 in pig retina |
| 28.8 (HLM) | 5.5 ± 0.4 in rabbit cornea |
a
HLM, human liver microsomes; rhCES, recombinant human CES1 or CES2.