(A) CV of CPE/BSA fully modified, contains the P.
pachyrhizi soybean rust spores, the aptamer, and the
AP enzyme: curve (a) in CBS. Curves (b–e) after the addition
of p-nitrophenyl phosphate substrate (2 mg in CBS,
5 mL) at different time intervals: 0, 5, 10, and 15 min, respectively.
Scan rate, 100 mV·s–1. (B) Expanded dotted
area of (A). (C) CV of CPE/BSA: curve a in CBS. Curves (be) after
the addition of p-nitrophenyl phosphate substrate
(2 mg in CBS, 5 mL) at different time intervals: 0, 5, 10, and 15
min, respectively. Scan rate, 100 mV·s–1. (D)
Expanded dotted area of (C).