Figure 2.

Human carcinoembryonic antigen (CEACAM5_HUMAN) sequence. Putative N-glycosylation sites are indicated in bold red. Glycopeptides that were detected after specific proteases digestion are highlighted in green (Glu-C) and orange (trypsin). The glycopeptide coverage by a nonspecific protease (Pronase) digestion is shown in blue. The total molecular mass of the glycosylated protein is reported to vary between 150 000 and 200 000 Da; the mass calculations are based on the most prominent mass (180 000 Da).²¹