Fig. 4.

HDNs in APS patients compared with healthy controls

HDNs isolated from 20 healthy controls and 20 patients with APS at baseline (immediately fixed after isolation), untreated and after stimulation with IO and PMA. (A) MFI of CD66b and (B) CD11b as well as (C) % of CD66b high and (D) % of CD11b high of healthy controls (grey boxes) and patients with APS (black boxes) after treatment for 30 min at 37°C. (E) MFI of H3Cit after stimulation for 3 h at 37°C. Statistical analysis was performed using Mann–Whitney U test. H3Cit: citrullinated histone H3; HDN: high density neutrophil; IO: ionomycin; MFI: mean fluorescent intensity; n.s.: not significant; PMA: phorbol 12-myristate 13-acetate. *P ≤0.05; **P ≤0.01; ***P ≤0.001.