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. 2021 Feb 26;10:e62474. doi: 10.7554/eLife.62474

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© 2021, Souque et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (a) Schematic representation of the experimental evolution protocol. (b) Top: Representation of the WTA3 integron. Bottom: Survival curves of the PA01:WTA3 and PA01:ΔintI1A3 populations during ramping treatment, monitored using OD595. The black triangles represent time points where populations were sequenced using whole genome sequencing. (c) Average minimum inhibitory concentration (MIC) of a random subset of the PA01:WTA3 (15 populations) and PA01:ΔintI1A3 populations (five populations) from the final ×1024 MIC time point. The MIC of the ancestral populations is represented by a dashed line. Error bars represent standard deviation. The MIC of each population was averaged from three biological replicates.