Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Pseudomonas aeruginosa)	PA01	Lab strain	NC_002516
Strain, strain background (Escherichia coli)	MG-1	Poirel et al., 1999	AF205943	E. coli clinical isolate containing a qacI–aadB–aacA1/orfG–blaVEB1–aadB–arr2–cmIA5–blaOXA–10/aadA1 integron array
Strain, strain background (Escherichia coli)	EIEC-4	Gassama et al., 2004		E. coli clinical isolate containing a dfrA5 integron cassette
Recombinant DNA reagent	R388	Avila and de la Cruz, 1988	NC_028464.1
Recombinant DNA reagent	WTA1	This study		Custom integron array drfA5–blaVEB1–aadB on R388 plasmid backbone
Recombinant DNA reagent	WTA2	This study		Custom integron array blaVEB1– aadB–dfrA5 on R388 plasmid backbone
Recombinant DNA reagent	WTA3	This study		Custom integron array blaVEB1–dfrA5–aadB on R388 plasmid backbone
Recombinant DNA reagent	WTA4	This study		Custom integron array dfrA5–aadB–blaVEB1 on R388 plasmid backbone
Recombinant DNA reagent	WTA5	This study		Custom integron array aadB–blaVEB1– dfrA5 on R388 plasmid backbone
Recombinant DNA reagent	WTA6	This study		Custom integron array aadB–dfrA5–blaVEB1 on R388 plasmid backbone
Recombinant DNA reagent	Δint1A3	This study		Array WTA3 with a 948 bp deletion of the integrase intI1
Recombinant DNA reagent	pAMBL1	San Millan et al., 2015a	KP873172.1	Clinical plasmid containing a blaVIM-1–aadB integron array
Software, algorithm	breseq	Barrick et al., 2014	RRID:SCR_010810	Version 0.33.2
Software, algorithm	CNOGpro	Brynildsrud, 2018