Schematics of microvascular network conditions for optimization of self-assembly of microvasculature (a), where (i) iPSC-ECs only, (ii) iPSC-ECs + PCs, (iii) iPSC-ECs + PCs + ACs. Confocal images of microvascular networks after 7 days (b), where (i) iPSC-ECs only (CD31, green), (ii) co-culture with PCs (F-actin, red), (iii) tri-culture with ACs [Glial fibrillary acidic protein (GFAP), magenta]. Scale bars indicate 100 μm. Reproduced with permission Campisi et al., Biomaterials 180, 117 (2018). Copyright 2018 Authors, licensed under a Creative Commons Attribution (CC BY) license.