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(A) Tumor cells negative for IDH1 R132H mutant protein (immunoperoxidase, ×400). (B) Tumor cells show loss of nuclear expression of ATRX (mutant phenotype) (immunoperoxidase, ×200). (C) Strong and diffuse immunopositivity for p53 protein (mutant phenotype) (immunoperoxidase, ×200). (D) Tumor cells show retained expression of H3K27me3 indicating absence for H2K37M mutant protein (immunoperoxidase, ×200).
