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. 2020 Aug 21;35(4):1073–1086. doi: 10.1038/s41375-020-01025-z

Fig. 5. The role of PHB2 in BCR pathway inhibitory effects of SLAMF1 and SLAMF7 receptors in CLL.

Fig. 5

a Representative WB analysis of PHB2 levels 96 h after siRNA transfection as indicated in the MEC-1 sublines. b Calcium flux was assessed 96 h after siRNA transfection of MEC-1 sublines baseline and c after IgM crosslinking. N = 6. d Co-immunoprecipitation of PHB2 in IgM and IgG-switched MEC-1 cells. Raji and Ramos cell lysates were used as a positive control for CD79a. For Western Blots, anti-CD79a and -PHB2 antibodies were used. Signal intensities of two independent western blots were quantified using ImageJ. e Proliferation was assessed in IgG-switched MEC-1 cells overexpressing SLAMF1 or SLAMF7 compared to empty vector control line after 120 h, N = 9. f Changes in calcium-flux after IgG crosslinking were assessed in IgG-switched MEC-1 cells overexpressing SLAMF1 or SLAMF7, N = 6. g Representative Western Blot analysis for Akt and Erk phosphorylation after stimulation with 10 µg/ml anti-IgM/IgG for 5 min in IgM or IgG-swiched MEC-1 cells overexpressing SLAMF1 or SLAMF7 compared to empty vector. Data from independent experiments are shown as mean, error bars represent SEM, statistical significance was calculated by one-way ANOVA and Bonferroni’s post-hoc test. WB Western blot, SEM standard error of the mean, FC flow cytometry.