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. 2021 Apr 6;12:2069. doi: 10.1038/s41467-021-22258-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The Chl a growth (a) and light microscopic pictures (b, d, e) of Pseudanabaena sp. PCC7367 cultures after the addition of Fe(II) or green rust (GR, c). Chl a growth curves (a) of cultures in an anoxic open-culture system with the addition of 600 µM Fe(II) (open, green), 100 µM GR (open, yellow), 200 µM GR (open, orange) or 400 µM GR (open brown) on day 4 of exponential growth. The positive control had 600 µM Fe(II) added at the start of the experiment (closed, green), while the negative control had no addition of Fe (closed, grey). On day 6, 2 days after the addition of Fe(II)/GR, light microscopic pictures were taken of the negative control culture (b), the GR in an abiotic control (c), the culture with the addition of 400 µM GR on day 4 (d) and the positive control with 600 µM Fe(II) at the start of the experiment (e). On day 9, the remaining Fe(II)/GR in the positive control was oxidised by exposure to oxic ambient air for 10 min. Data are presented as mean values ± SD with n = 3 biologically independent samples. Light microscopic images were taken at 100x magnification and are presented with a 10 µm scale bar. Asterisks (*) represent a significant difference between Fe(II)/GR supplemented cultures and the growth control (p < 0.05, Student’s t-test, two-tailed).