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. 2021 Apr 6;12(4):357. doi: 10.1038/s41419-021-03644-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Three lineage differentiation of huMSCs and expression of cell surface markers. Upper Panel from left to right: MSCs differentiated to adipocytes, chondrocytes, and osteocytes. B BV2-suppression capacity and proliferation capacity of 32 lines of huMSCs. The black bars represent the ratio of BV2 cell mass after 48 h cultured in MSC-CM compared to RPMI-1640 (control medium). The red dots represent the ratio of total huMSC mass at 72 h after inoculation compared to 1 h after inoculation. C correlation between BV2-suppression capacity and huMSC proliferation capacity of 32 huMSC lines. D Boxplot shows BV2-suppression capacity between male (n = 20) and female (n = 20) huMSC lines, eight extra huMSC lines were added to increase the statistical power.