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. 2021 Apr 6;12:2074. doi: 10.1038/s41467-021-22205-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b The experimental design of the chemogenetic activation of Trpa1⁺ neurons projecting to the NTS (a) and representative retrograde-labeled mCherry expression in the VG in Trpa1-Cre and control mice (b). c Representative images of medulla (left), magnified views in the NTS (middle), and quantification (right) of c-fos⁺ cells in the NTS after CNO administration are shown (n = 8 for Trpa1-Cre⁺/sal and Trpa1-Cre⁺/CNO and n = 6 for Trpa1-Cre⁻/CNO, p = 0.0135 between Trpa1-Cre⁺/sal and Trpa1-Cre⁺/CNO, and p = 0.5335 between Trpa1-Cre⁺/sal and Trpa1-Cre⁻/CNO). d, e Temporal and mean cutaneous temperature after administration of saline (black) and CNO (red) are shown for hM3Dq-infected Trpa1-Cre (d; n = 7 each, p = 0.0003) and control mice (e; n = 6 each, p = 0.1562). Data are shown as mean ± SEM. Kruskal–Wallis with Dunn’s multiple test (c), one-tailed, Mann–Whitney U test (d), and one-tailed, Wilcoxon test (e) were used to assess significance; *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars, 100 µm.