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. 2021 Apr 6;11:7545. doi: 10.1038/s41598-021-86860-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of calcein uptake by SKBR3 and MDA-MB-231 cells following their incubation with control liposomes or TRA-liposomes followed by ultrasound (US) sonication (35-kHz) for 5 min at a power density of 20 mW/cm². Untreated cells served as a negative control for background fluorescence. An average of 1 × 10⁴ cells was analyzed from each sample with calcein fluorescence intensity showed on a four-decade log scale.