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. 2021 Apr 6;12(4):356. doi: 10.1038/s41419-021-03626-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Binding sites for EIF4A3 on the CSNK1G1 mRNA transcript, upstream or downstream of hsa_circ_101555, obtained from Circular RNA Interactome. B Pull-down assay confirms the enrichment of EIF4A3 protein in CSNK1G1 mRNA pull-down. C Quantitative real-time PCR following EIF4A3-RIP assay confirms the binding of EIF4A3 on the predicted binding region (a, b, c, and d) of CSNK1G1 mRNA. H19, a known interacting long noncoding RNA (lncRNA) with EIF4A3, served as the positive control. The intron 10 of CSNK1G1 mRNA was used as a negative control. D HCCLM3 and HepG2 cells transfected with control or siRNA targeted eIF4A3; hsa_circ_101555 expression detected by qRT-PCR. E HCCLM3 and HepG2 cells transfected with control or eIF4A3 overexpression plasmids; hsa_circ_101555 expression measured by qRT-PCR. F HCCLM3 and HepG2 cells transfected with control or siRNA targeted hsa_circ_101555; eIF4A3 expression detected by qRT-PCR.