Table 1.

mAb characterization by isotyping, SPR and alanine scan analysis.

Target	Hybridoma	Isotype	ka (M−1s−1)	kd (s−1)	KD (M)	Critical residues
AG1	30D12	IgG1/kappa	4.51 × 105	2.05 × 10−5	4.55 × 10−11	TGKKNGNGEAGEFLPEDFRDGEYE
	8A3	IgM/kappa	2.08 × 105	3.69 × 10−5	1.77 × 10−10	TGKKNGNGEAGEFLPEDFRDGEYE
AG4	13E10	IgG1/kappa	NB	NB	NB	NB
	4E1	IgG1/kappa	NB	NB	NB	NB
	10B12	IgG2A/kappa	7.28 × 105	1.26 × 10−4	1.73 × 10−10	LSDKNNPDVCDEY
	5C1	IgG1/kappa	3.07 × 105	3.56 × 10−5	1.16 × 10−10	LSDKNNPDVCDEY
	1B1	IgG1/kappa	3.09 × 105	3.13 × 10−5	1.01 × 10−10	LSDKNNPDVCDEY
AG5	4F2	IgG1/kappa	6.68 × 105	1.84 × 10−5	2.76 × 10−11	DLNAKDREGDTPLH
	27D7	IgG1/kappa	7.05 × 105	1.23 × 10−4	1.74 × 10−10	DLNAKDREGDTPLH
	2C9	IgG2A/kappa	4.47 × 105	8.14 × 10−5	1.82 × 10−10	DLNAKDREGDTPLH
	6A4	IgG1/kappa	8.72 × 105	3.33 × 10−5	3.82 × 10−10	DLNAKDREGDTPLH
	11D11	IgG1/kappa	1.00 × 106	1.33 × 10−5	1.33 × 10−11	DLNAKDREGDTPLH

Critical residues (highlighted in bold underline) are defined as those that reduce the ELISA colorimetric response to <50% of the wild-type sequence (Supplementary Fig. 4).

ka association constant, kd dissociation constant, KD equilibrium constant, NB no binding.